January 17th, 2014

Today we will complete the microinjections for group D. The rats in D3 received .05 and .3 NaCl. The aCSF microinjections went smoothly. However, both CDP rats had to be redone (one in both cannulae, the other in just the left cannula). Bekah and I flipped the water bottles for the rats in group C for an hour. We experienced some difficulties during the second round of microinjections (D1), where the rats received received saccharin. The tubing was not cooperating for the acsf or the cdp rats. Bekah and I agitated the cannulae for the rats in A and B. Hopefully, the third set of microinjections will go smoothly for the afternoon group. Tomorrow, I will come in and flip the water bottles for the rats for an hour.