January 14th, 2014

This morning we completed microinjections for three groups of rats (Group C: 1, 2, 3). The schedule is much more complicated, as we have added MSG and saccharin solutions. As a result, we all had to be much more vigilant when checking the solutions during the microinjections. We also needed to make sure that we double checked the amount of fluid required for the solution, as some of them need 400ml of DI water as opposed to 200ml. Group C's injections went smoothly. However, we needed to redo one of the injections for two rats. It seems that the cannulae are getting stopped up. After the microinjections, Bekah and I agitated the cannulae for the rats in group D. Two of the rats in group D have clogged cannulae and Sarah had to rinse the cannulae with ethanol. Tomorrow will definitely be a busy day!