January 20th, 2014

Today, I began the day by making up .03 NaCL solution. We got off to a slow start in the first round of microinjections on C2, where the rats received quinine. Although the aCSF microinjections went smoothly, the CDP injections proved to be more problematic. Afterward, Bekah and I agitated the cannulae and weighed the rats in A and B. The aCSF microinjections also went smoothly for the rats in C3. However, we had to redo the microinjections for the CDP rats. The rats in boxes 1-3 received quinine, whereas the rat in box 4 received NaCl. We also prepared the LiCl solutions for the CTA tests. Dr. Holstein also explained the perfusion process and what to expect. The microinjections for the C3 group, receiving quinine, were successful and we did not need do redo any of them!