January 23rd, 2014

Today I began the day by setting up the computer. The first round of injections (D1- all receiving quinine) went smoothly for the aCSF. However, we needed to redo the left injection for the first CDP rat. After Bekah and I flipped the water for group C, we agitated the cannulae for the rats in A and B groups. Dr. Holstein was concerned that the cannulae could be clogged, which could be problematic when they inject the dye for the perfusions. The second round of injections also went well for D2 (all receiving quinine). Similar to the first set of injections, we needed to redo the left cannulae for the CDP rats. We also labeled the bottles for the brains. D3 also went smoothly. However, we had to redo the left cannula for the rat in box two and three.